Anest. intenziv. Med. 2018;29(6):322-327

Administration of 500 ml of normal saline does not result in immediate damage to the endothelial glycocalyx determinable by sublingual microcirculation assessmentCelková anestezie - původní práce

J. Pouska^1,2,*, D. Astapenko^3,4, V. Tégl^1,2,5, J. Beneš^1,2,5, V. Černý^3,4,6,7,8,9

¹ Klinika anesteziologie, resuscitace a intenzivní medicíny, Fakultní nemocnice Plzeň

² Klinika anesteziologie, resuscitace a intenzivní medicíny, Lékařská fakulta v Plzni, Univerzita Karlova

³ Klinika anesteziologie, resuscitace a intenzivní medicíny, Fakultní nemocnice Hradec Králové

⁴ Lékařská fakulta v Hradci Králové, Univerzita Karlova

⁵ Biomedicínské centrum, Lékařská fakulta v Plzni, Univerzita Karlova

⁶ Klinika anesteziologie, perioperační a intenzivní medicíny, Univerzita J. E. Purkyně, Masarykova nemocnice, Ústí nad Labem

⁷ Institut postgraduálního vzdělávání ve zdravotnictví

⁸ Centrum pro výzkum a vývoj, Fakultní nemocnice Hradec Králové

⁹ Dept. of Anesthesia, Pain Management and Perioperative Medicine, Dalhousie University, Halifax, Kanada

Objective:
Assessment of endothelial glycocalyx alteration after infusion of 500 ml of normal saline by perfused boundary region (PBR) parameter in twelve healthy volunteers.


Hypothesis:
Endothelial glycocalyx will be significantly altered by infusion of 500 ml of normal saline.

Design:
Non-randomized, prospective, double-centre study.


Settings:
Two ICUs in University Hospitals.

Materials and methods:
Twelve healthy volunteers without acute illness were recruited. 500 ml of normal saline was infused to each participant. We collected basic haemodynamic data as well as perfused boundary region as a marker of endothelial glycocalyx integrity before and after the fluid administration. Student T-test (paired) with p-value of 0.05 was used for the statistical analysis.

Results:
After administration of 500 ml of normal saline there was non-significant change in PBR (2.00±0.21 vs. 2.21±0.36, p = 0.089). Concerning the macrohaemodynamics, there was a significant drop in the heart rate after the infusion (74±10 vs. 68±10, p = 0.003).

Conclusion:
Our study did not confirm alteration of the endothelial glycocalyx after intravenous administration of 500 ml of normal saline to healthy volunteers. There was a significant decrease of heart rate after the infusion but no other changes were observed.

Keywords: microcirculation; intravenous infusion; endothelial glycocalyx

Received: July 23, 2018; Accepted: November 1, 2018; Published: December 1, 2018  Show citation