Anest. intenziv. Med. 2008;19(1):5-8

Some aspects of molecular biology in critically ill patients in the context of current laboratory medicineIntensive Care Medicine

M. Průcha*, S. Peková, L. Sedláčková
Oddělení klinické biochemie, hematologie a imunologie, Nemocnice Na Homolce, Praha

Biomarkers are recognized as objective indicators of normal biological processes, pathogenic processes or pharmacological responses to a therapeutic intervention.
Sepsis is a multifactorial disease distinguished by the paucity of diagnostic tests (biomarkers) available. New biomarkers allow for a better understanding of the pathogenesis of diseases and better stratification of patients in connection with improved clinical decision making, diagnosis and treatment. New technologies - genomics and proteomics - enable multiplex detection and evaluation of biomarkers. The goal of the diagnostics at present is the implementation of new technologies and molecular approaches into routine clinical practice with emphasis on therapeutic consequences that could make "personalized medicine" a real option.

Keywords: biomarkers; genomics; molecular biology; proteomics; sepsis

Published: February 1, 2008  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Průcha M, Peková S, Sedláčková L. Some aspects of molecular biology in critically ill patients in the context of current laboratory medicine. Anest. intenziv. Med. 2008;19(1):5-8.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Marshall, J. C. Such stuff as dreams are made on: mediator - directed therapy in sepsis. Nature Rev. Drug Discovery, 2003, 2, p. 391-405. Go to original source... Go to PubMed...
    2. Beutler, B., Hoebe, K., Du, X., Ulevitch, R. J. How we detect microbes and respond to them: the Toll-like receptors and their transducers.
    3. Volk, H. D., Reinke, P., Kraus, D. et al. Monocyte deactivation - rationale for a new therapeutic strategy in sepsis. Intensive Care Med., 1996, 22, Suppl. 4, p. 474-481. Go to original source... Go to PubMed...
    4. Sedláčková, L., Průcha, M., Dostál, M. Immunological monitoring of sepsis using flow citometry - quantitation of monocyte HLA-DR expression and granulocyte CD64 expression. Epidemiol. Mikrobiol. Imunol., 2005, 54, s. 54-61. Go to PubMed...
    5. Wesche, D. E., Lomar-Neira, J. L., Perl, M. et al. Leukocyte apoptosis and its significance in sepsis and shock. J. Leukocyte Biology, 2005, 78, p. 1-13. Go to original source... Go to PubMed...
    6. Hong, J., Lejny, E., Fraser, A. G. et al. TLR2, TLR4 and TLR9 polymorphisms and Crohn's disease in a New Zealand Caucasian cohort. J. Gastroenterol. Hepatol., 2007, 22, 11, p. 1760-1766. Go to original source... Go to PubMed...
    7. Baccala, R., Hoebe, K., Kono, D. H. et al. TLR-dependent and TLR-independent pathways of type I interferon induction in systemic autoimmunity. Nat. Med., 2007, 13, 5, p. 543-551. Go to original source... Go to PubMed...
    8. Tang, A. H., Brunn, G. J., Cascalho, M., Platt, J. L. Pivotal advance: Endogenous pathway to SIRS, sepsis, and related conditions. J. Leucocyte Biology, 2007, 82. Go to original source... Go to PubMed...
    9. Eichacker, P. Q., Parent, C., Kalil, A. et al. Risk and the efficacy of antiinflammatory agents: retrospective and confirmatory studies of sepsis. Am. J. Respir. Crit. Care Med., 2002,166, p. 1197-1205. Go to original source... Go to PubMed...
    10. Toft, P. The effect of surgical stress, endotoxin induced sepsis and extracorporeal circulation on the cell mediated immunity. Dan. Med. Bull., 2002, 49, 4, p. 302-317.
    11. Simon, L., Gauvin, F., Amre, D. K. et al. Serum procalcitonin and C-reactive protein levels as markers of bacterial infection: a systematic review and meta-analysis. Clin. Infect. Dis., 2004, 15, 39, 2, p. 206-217. Go to original source... Go to PubMed...
    12. Oberholzer, A., Sousa, S. M., Tschoeke, S. K. et al. Plasma cytokine measurements augment prognostic scores as indicators of outcome in patients with severe sepsis. Shock, 2005, 23, 6, p. 488-493. Go to PubMed...
    13. Davis, B. H. Improved diagnostic approaches to infection/sepsis detection. Expert. Rev. Mol. Diagn., 2005, 5, p. 193-207. Go to original source... Go to PubMed...
    14. Kellum, J. A., Kong, L., Fink, M. P. et al. Understanding the inflammatory cytokine response in pneumonia and sepsis. Results of the genetic and inflammatory markers of sepsis (GenIUS) Study. Arch. Intern. Med., 2007, 35, p. 1061-1067. Go to original source... Go to PubMed...
    15. Průcha, M., Maťoška, V. PIRO systém "P" (predispozice) a sepse. Anesteziologie a intenzivní medicína, 2005, 4, s. 203-210.
    16. Sterus, T., Pohlak, N., Echtenacher, B., Mannel, D. N. Divergence of protection induce by bacterial products and sepsis-induced immune suppression. Infect. Immun., 2005, 43, p. 4905-4912. Go to original source... Go to PubMed...
    17. Kollef, M. H. The importance of appropriate initial antibiotic therapy for hospital-acquired infections. Am. J. Med., 2003, 115, 7, p. 582-584. Go to original source...
    18. Chuang, T. P., Laramie, J. M., Meyer, D. J. et al. Molecular diagnostics in sepsis: from bedside to bench. J. Am. Coll. Surg., 2006, 203, p. 585-598. Go to original source... Go to PubMed...

    Return to the content


    Anesteziologie a intenzivní medicína

    Madam, Sir,
    please be aware that the website on which you intend to enter, not the general public because it contains technical information about medicines, including advertisements relating to medicinal products. This information and communication professionals are solely under §2 of the Act n.40/1995 Coll. Is active persons authorized to prescribe or supply (hereinafter expert).
    Take note that if you are not an expert, you run the risk of danger to their health or the health of other persons, if you the obtained information improperly understood or interpreted, and especially advertising which may be part of this site, or whether you used it for self-diagnosis or medical treatment, whether in relation to each other in person or in relation to others.

    I declare:

    1. that I have met the above instruction
    2. I'm an expert within the meaning of the Act n.40/1995 Coll. the regulation of advertising, as amended, and I am aware of the risks that would be a person other than the expert input to these sites exhibited

    No
    Yes

    If your statement is not true, please be aware
    that brings the risk of danger to their health or the health of others.